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Rabbit Anti-LSP1  antibody (bs-5154R)  
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產品編號 bs-5154R
英文名稱 Rabbit Anti-LSP1  antibody
中文名稱 白細胞F肌動蛋白結合蛋白抗體
別    名 47 kDa actin binding protein; 47 kDa actin-binding protein; 52 kDa phosphoprotein; F actin binding and cytoskeleton associated protein; Leufactin (leukocyte F-actin binding protein); Leufactin; Leukocyte F actin binding protein; Leukocyte specific protein 1; LSP 1; Lsp1; LSP1_HUMAN; Lymphocyte specific antigen WP34; Lymphocyte specific protein 1; Lymphocyte specific protein pp52; Lymphocyte-specific antigen WP34; Lymphocyte-specific protein 1; pp52; Protein pp52; S37 protein; WP 34; WP34.    OTTHUMP00000014138; OTTHUMP00000014139; OTTHUMP00000014140; OTTHUMP00000069612; OTTHUMP00000069614; OTTHUMP00000069615; OTTHUMP00000069616;
研究領域 細胞生物  免疫學  結合蛋白  淋巴細胞  t-淋巴細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse (predicted: Rat,Dog)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 37kDa
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human LSP1: 241-339/339 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Function:
May play a role in mediating neutrophil activation and chemotaxis (By similarity).

Subunit:
Binds actin.

Subcellular Location:
Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity:
Activated T-lymphocytes.

Post-translational modifications:
Phosphorylated by casein kinase II, protein kinase C and MAPKAPK2. Phosphorylation by PKC induces translocation from membrane to cytoplasm. Phosphorylation by MAPKAPK2 may regulate neutrophil chemotaxis (By similarity).

SWISS:
P33241

Gene ID:
4046

Database links:

Entrez Gene: 4046 Human

Entrez Gene: 16985 Mouse

Entrez Gene: 361680 Rat

Omim: 153432 Human

SwissProt: P33241 Human

SwissProt: P19973 Mouse

Unigene: 56729 Human

Unigene: 234003 Mouse

Unigene: 101608 Rat



產品圖片
Sample: Uterus (Mouse) Lysate at 40 ug Primary: Anti- LSP1 (bs-5154R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 54 kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-LSP1 Polyclonal Antibody, Unconjugated(bs-5154R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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