| 產(chǎn)品編號(hào) | bs-4066R |
| 英文名稱 | Rabbit Anti-phospho-CREM (Ser271 + Ser274) antibody |
| 中文名稱 | 磷酸化環(huán)磷酸腺苷反應(yīng)元件調(diào)節(jié)蛋白抗體 |
| 別 名 | CREM (phospho S271 + S274); p-CREM (phospho S271 + S274); cAMP response element modulator; cAMP responsive element modulator; hCREM 2; hCREM2; ICER; Inducible cAMP early repressor; Inducible cAMP early repressor ICER; MGC111110; MGC17881; MGC41893. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 結(jié)合蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Chicken,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37kDa |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human CREM around the phosphorylation site of Ser271 + Ser274: AA(p-S)PG(p-S)LH |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
CREM is a a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is derived from a multiexonic gene that encodes both activators and antagonists of cAMP-inducible transcription by differential splicing. Splice variants with antagonistic function lack 2 glutamine-rich domains and block cAMP-induced transcription, whereas an isoform that includes these glutamine-rich domains is a transcriptional activator. Function: Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation. Subunit: Binds DNA as a dimer. Interacts with FHL5. Interacts with CDC34. Subcellular Location: Nucleus. Tissue Specificity: Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes. Post-translational modifications: Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome. Stimulated by phosphorylation. Similarity: Belongs to the bZIP family. Contains 1 bZIP domain. Contains 1 KID (kinase-inducible) domain. SWISS: Q03060 Gene ID: 1390 Database links: Entrez Gene: 1390 Human Entrez Gene: 12916 Mouse Omim: 123812 Human SwissProt: Q03060 Human SwissProt: P27699 Mouse Unigene: 200250 Human Unigene: 10251 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CREM (Ser271 +Ser 274)) Polyclonal Antibody, Unconjugated (bs-4066R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CREM (Ser271 +Ser 274)) Polyclonal Antibody, Unconjugated (bs-4066R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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