| 產品編號 | bs-5127R |
| 英文名稱 | Rabbit Anti-Annexin A10 antibody |
| 中文名稱 | 膜粘連蛋白10抗體 |
| 別 名 | annexin 14; Annexin A10; Annexin-10; Annexin-14; ANX 14; ANX10_HUMAN; ANX14; ANXA 10; ANXA10. |
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Specific References (1) | bs-5127R has been referenced in 1 publications.
[IF=2.11] Lu, Ying, et al. "Annexin A10 is involved in the development and maintenance of neuropathic pain in mice." Neuroscience Letters (2016). WB, IHC-F ; Mouse.
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| 研究領域 | 細胞生物 免疫學 信號轉導 結合蛋白 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 37kDa |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Annexin A10: 251-324/324 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. The function of this gene has not yet been determined. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: Q9UJ72 Gene ID: 11199 Database links: Entrez Gene: 11199 Human Entrez Gene: 26359 Mouse Omim: 608008 Human SwissProt: Q9UJ72 Human SwissProt: Q9QZ10 Mouse Unigene: 188401 Human Unigene: 42179 Mouse Unigene: 101584 Rat |
| 產品圖片 |
Sample:
A549(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- Annexin A10 (bs-5127R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 37 kD
Blank control:A549.
Primary Antibody (green line): Rabbit Anti-Annexin A10 antibody (bs-5127R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 5,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
