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Rabbit Anti-ATG16L  antibody (bs-4007R)  
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產品編號 bs-4007R
英文名稱 Rabbit Anti-ATG16L  antibody
中文名稱 自噬相關蛋白16A抗體
別    名 A16L1_HUMAN; APG16 like 1; APG16-like 1; APG16L; APG16L beta; ATG16A; ATG16 autophagy related 16 like 1; ATG16 autophagy related 16-like 1 (S. cerevisiae); ATG16 autophagy related 16-like 1; ATG16A; ATG16L; ATG16L1; Autophagy related protein 16 1; Autophagy-related protein 16-1; FLJ00045; FLJ10035; FLJ10828; FLJ22677; IBD10; OTTHUMP00000164391; OTTHUMP00000164393; OTTHUMP00000165876; OTTHUMP00000165877; WD repeat domain 30; WDR30.  
Specific References  (1)     |     bs-4007R has been referenced in 1 publications.
[IF=0] Haider F. Ghazi et al. Immunohistochemical Expression of Xenophagy Proteins in Helicobacter pylori and None Helicobacter pylori Gastritis. J Pure Appl Microbiol,2018 12(4), 1795-1800 Dec. 2018  IHC ;  Human.  
研究領域 腫瘤  細胞生物  免疫學  神經生物學  信號轉導  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 68kDa
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ATG16A: 501-607/607 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.

Function:
Plays an essential role in autophagy (By similarity).

Subunit:
Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa).

Subcellular Location:
Cytoplasm (By similarity). Preautophagosomal structure membrane; Peripheral membrane protein (By similarity). Note=Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5 (By similarity).

DISEASE:
Genetic variations in ATG16L1 are associated with susceptibility to inflammatory bowel disease type 10 (IBD10) [MIM:611081]. IBD is characterized by a chronic relapsing intestinal inflammation. IBD is subdivided into Crohn disease (CD) and ulcerative colitis phenotypes. IBD10 individuals show the phenotype characteristic to CD. It may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. CD is commonly classified as autoimmune disease.

Similarity:
Belongs to the WD repeat ATG16 family.
Contains 7 WD repeats.

SWISS:
Q676U5

Gene ID:
55054

Database links:

Entrez Gene: 55054 Human

Entrez Gene: 77040 Mouse

Omim: 610767 Human

SwissProt: Q676U5 Human

SwissProt: Q8C0J2 Mouse

Unigene: 529322 Human

Unigene: 272972 Mouse



產品圖片
Sample: Lovo Cell (Human) Lysate at 30 ug Primary: Anti- ATG16L (bs-4007R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kD
Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATG16L Polyclonal Antibody, Unconjugated(bs-4007R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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