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Rabbit Anti-PLASTIN3  antibody (bs-3643R)  
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產品編號 bs-3643R
英文名稱 Rabbit Anti-PLASTIN3  antibody
中文名稱 絲束蛋白T Plastin抗體
別    名 Plastin 3 (T isoform); PLS3; PLS 3; PLS-3; Plastin 3; Plastin3; Plastin-3; T fimbrin.  
研究領域 細胞生物  免疫學  細胞分化  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 69kDa
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PLASTIN3: 401-500/630 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 T Plastin is a representative of an important family of actin-cross-linking and -bundling proteins that organize most of the actin networks in cells. T Plastin is associated with the actin core bundle of microvilli, stereocilia and microspikes in epithelial cells and was proposed to participate in the assembly of these structures. In mesenchymal cells, T plastin localizes to focal adhesions and the leading edge, a broad lamellar membrane extension harboring a dynamic branched actin network. Recent studies have shown that T plastin has the potential to be a Sezary cell-specific marker valuable for diagnostic and treatment of Sezary syndrome.

Function:
Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.

Subunit:
Monomer.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Expressed in a variety of organs, including muscle, brain, uterus and esophagus.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR

Similarity:
Contains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains.

SWISS:
P13797

Gene ID:
5358

Database links:

Entrez Gene: 5358 Human

Entrez Gene: 102866 Mouse

Entrez Gene: 81748 Rat

Omim: 300131 Human

SwissProt: P13797 Human

SwissProt: Q99K51 Mouse

SwissProt: Q63598 Rat

Unigene: 496622 Human

Unigene: 28777 Mouse

Unigene: 32103 Rat



絲束(纖毛素)是肌動蛋白結合蛋白家族的成員,存在于細胞支架的重組中. 絲束3是來源于纖毛素蛋白家族的T淋巴細胞特異肌動蛋白成束蛋白。近年來T Plastin在用于sezary cell綜合征的研究方面較多。
產品圖片
Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-PLASTIN3 (bs-3643R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kD Observed band size: 69 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLASTIN3) Polyclonal Antibody, Unconjugated (bs-3643R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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