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Rabbit Anti-Fbx32  antibody (bs-2591R)  
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產品編號 bs-2591R
英文名稱 Rabbit Anti-Fbx32  antibody
中文名稱 泛素蛋白連接酶抗體
別    名 Fbx32; 4833442G10Rik; AI430017; Atrogin 1; Atrogin-1; ATROGIN1; Atrophy gene 1; F box only protein 32; F-box only protein 32; F-box protein 32; FBX32_HUMAN; fbxo25; FBXO32; FLJ32424; MAFbx; MGC108443; MGC137646; MGC33610; Muscle atrophy F box; Muscle atrophy F box protein; Muscle atrophy F-box protein.  
Specific References  (3)     |     bs-2591R has been referenced in 3 publications.
[IF=6.684] Ma X. et al. Endoplasmic Reticulum Stress Is Involved in Muscular Pathogenesis in Idiopathic Inflammatory Myopathies.. Front Cell Dev Biol. 2022 Feb;10:791986-791986  IHC ;  Human.  
[IF=5.62] Lin, Xuzhu, et al. "Hindlimb Immobilization, but not Castration, Induces Reduction of Undercarboxylated Osteocalcin Associated with Muscle Atrophy in Rats." Journal of Bone and Mineral Research (2016).  WB ;  Rat.  
[IF=3.412] Zhang Quan-Bing. et al. Effect of Electrical Stimulation on Disuse Muscular Atrophy Induced by Immobilization: Correlation with Upregulation of PERK Signal and Parkin-Mediated Mitophagy. AM J PHYS MED REHAB. 2023 Jan;:10.1097/PHM.0000000000002182  IHC ;  Rabbit.  
研究領域 細胞生物  干細胞  泛素  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Dog,Horse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=0.2ug/test,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 42kDa
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MAFbx: 31-130/355 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Fbx32 is an E3 ubiquitin ligase that initiates ATP dependent ubiquitin-mediated proteolysis and promotes muscle atrophy. It is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. It is also thought to recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation during skeletal muscle atrophy. Fbx32 interacts with MyoD by ubiquitination via a sequence found in transcriptional coactivators and therefore may play an important role in the course of muscle differentiation by determining the abundance of MyoD.

Function:
Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.

Subunit:
Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32.

Subcellular Location:
Cytoplasm. Nucleus. Note=Shuttles between Cytoplasm and the nucleus.

Tissue Specificity:
Specifically expressed in cardiac and skeletal muscle.

Similarity:
Contains 1 F-box domain.

SWISS:
Q969P5

Gene ID:
114907

Database links:

Entrez Gene: 513776 Cow

Entrez Gene: 114907 Human

Entrez Gene: 67731 Mouse

Entrez Gene: Q2KHT6 Pig

Entrez Gene: 171043 Rat

Omim: 606604 Human

SwissProt: Q2KHT6 Cow

SwissProt: Q969P5 Human

SwissProt: Q9CPU7 Mouse

SwissProt: Q1A730 Pig

SwissProt: Q91Z62 Rat

Unigene: 403933 Human

Unigene: 292042 Mouse

Unigene: 72619 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Fbx32) Polyclonal Antibody, Unconjugated (bs-2591R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat uterus tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MAFbx/Fbx32 Polyclonal Antibody, Unconjugated(bs-2591R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Fbx32 antibody (bs-2591R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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