產品編號 | bs-1598R |
英文名稱 | Rabbit Anti-Cathepsin G antibody |
中文名稱 | 組織蛋白酶G抗體 |
別 名 | CTSG; Cathepsin G; CG; Cathepsin G precursor; CathepsinG; CTSG; CTSG protein; MGC23078; CATG_HUMAN. |
研究領域 | 細胞生物 免疫學 合成與降解 |
抗體來源 | Rabbit |
克隆類型 | Polyclonal |
交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Sheep,Cow,Dog,Horse) |
產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 28kDa |
細胞定位 | 細胞核 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CTSG: 41-120/255 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產品介紹 |
Cathepsin G is a serine protease, it consisting of a single polypeptide with a molecular weight of 30 kDa as determined by SDS-PAGE. Its substrates are collagen and proteoglycans. Poor regulation of Cathepsin G levels has been associated with various connective tissue disorders, for example pulmonary emphysema and cystic fibrosis. Function: Serine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride. Subcellular Location: Cell surface. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. SWISS: P08311 Gene ID: 1511 Database links: Entrez Gene: 1511 Human Omim: 116830 Human SwissProt: P08311 Human Unigene: 421724 Human |
產品圖片 |
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cathepsin G Polyclonal Antibody, Unconjugated(bs-1598R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |