| 產品編號 | bs-0448R |
| 英文名稱 | Rabbit Anti-NIS antibody |
| 中文名稱 | 鈉碘轉運體蛋白抗體 |
| 別 名 | Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na(+)/I(-) cotransporter; Na(+)/I(-) symporter; Na+/I- cotransporter; Na+/I-symporter; NIS; SC5A5_HUMAN; SLC5A5; sodium iodide symporter; Sodium-iodide symporter; Sodium/iodide cotransporter; Solute carrier family 5 (sodium iodide symporter) member 5; Solute carrier family 5; Solute carrier family 5 member 5. |
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Specific References (11) | bs-0448R has been referenced in 11 publications.
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| 研究領域 | 免疫學 通道蛋白 轉運蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NIS: 525-618/618 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
catalyzes Na+/I- symporter activity plays a role in iodide transport and thyroid hormone generation. Human Sodium Iodide Symporter (hNIS) is responsible for iodide concentrating ability within thyroid follicular cells. It is a membrane bound glycoprotein with 13 membrane spanning domains and 14 extramembranous domains. It may represent an autoantigen in thyroid. Function: Mediates iodide uptake in the thyroid gland. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Expression is primarily in thyroid tissue, but also to a lower extent in mammary gland and ovary. Expression is reduced in tumors. DISEASE: Thyroid dyshormonogenesis 1 (TDH1) [MIM:274400]: A disorder characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. SWISS: Q99PN0 Gene ID: 6528 Database links: Entrez Gene: 6528 Human Omim: 601843 Human SwissProt: Q92911 Human Unigene: 103983 Human Unigene: 584804 Human Unigene: 10505 Rat 鈉/碘轉運體蛋白-NIS，是位于甲狀腺細胞基底膜上的一類膜抗原，在甲狀腺對碘的主動轉運過程中發揮重要作用。 鈉/碘轉運蛋白受甲狀腺刺激素(TSH)、碘、細胞因子和生長因子等多種因素的廣泛調節。自身免疫性甲狀腺疾病時NIS的表達發生了明顯變化，患者體內出現抗NIS自身抗體。 此外，NIS還與甲狀腺冷結節和先天性甲狀腺機能減退等甲狀腺疾病的發病機理密切相關，近年來得到了足夠的重視。 |
| 產品圖片 |
Sample:
Lane 1: Mouse Stomach tissue lysates
Lane 2: Rat Stomach tissue lysates
Primary: Anti-NIS (bs-0448R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 48 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(bs-0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NIS Polyclonal Antibody, Unconjugated(bs-0448R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
