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Rabbit Anti-Nanog  antibody (bs-0829R)  
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產品編號 bs-0829R
英文名稱 Rabbit Anti-Nanog  antibody
中文名稱 胚胎干細胞關鍵蛋白抗體
別    名 NANOG_MOUSE; Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; FLJ40451; Homeobox transcription factor Nanog; Nanog homeobox; Homeobox protein NANOG; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog; Nanog; Ecat4.  
Specific References  (11)     |     bs-0829R has been referenced in 11 publications.
[IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin?Cancer?Res.?2019 Feb 21;38(1):93.  IF&WB ;  Human&Mouse.  
[IF=4.963] El-Badawy et al. Cancer cell-soluble factors reprogram mesenchymal stromal cells to slow cycling, chemoresistant cells with a more stem-like state. (2017) Stem.Cell.Res.Ther. 8:254  FCM&ICC ;  Human.  
[IF=3.44] Li X et al. A comparative study of the behavior of neural progenitor cells in extrusion-based in vitro hydrogel models. Biomed Mater. 2019 Sep 9;14(6):065001.  WB ;  Mouse.  
[IF=3.166] Liu et al. JNK is required for maintaining the tumor-initiating cell-like properties of acquired chemoresistant human cancer cells. (2015) Acta.Pharmacol.Si. 36:1099-106  WB ;  Human.  
[IF=3.143] Sung-Moo Lee. et al. Effect of the phenylpyrrole fungicide fludioxonil on cell proliferation and cardiac differentiation in mouse embryonic stem cells. Reprod Toxicol. 2021 Sep;104:76  WB ;  Mouse.  
[IF=2.649] Kim CW et al. Effects of cigarette smoke components on myocardial differentiation of mouse embryonic stem cells. Environ Toxicol. 2019 Sep 10.  ICF ;  Mouse.  
[IF=2.416] Lu, Yinglin. et al. Ascorbic acid and all-trans retinoic acid promote proliferation of chicken blastoderm cells (cBCs) by mediating DNA demethylation. In Vitro Cell Dev-An. 2022 Mar;:1-11  IF ;  Chicken.  
[IF=2.15] Liu, Yu-Hong, et al. "A signature for induced pluripotent stem cell–associated genes in colorectal cancer." Medical Oncology 30.1 (2013): 1-11.  WB ;  Human.  
[IF=1.571] Wu Q et al. Expression pattern Nanog is associated with the development of gestational trophoblastic neoplasia in Chinese women from Fujian province: a case-control study. Gynecol Endocrinol . 2020 Jun 25;1-5.  IHC-P ;  Human.  
[IF=1.461] Pei?W et al. Biological characterization and pluripotent identification of ovine amniotic fluid stem cells. Cytotechnology. 2018 Jun;70(3):1009-1021.  IF&FCM ;  ovine embryo.  
[IF=0] El-Badawy, Ahmed, et al. "Ovarian Extract Promotes Proliferation and Upregulates Pluripotency of Mesenchymal Stromal Cells." Journal of Ecology of Health & Environment (2016).  IF(ICC) ;  Mouse.  
研究領域 腫瘤  染色質和核信號  信號轉導  干細胞  細胞周期蛋白  細胞表面分子  細胞分化  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse (predicted: Rat,Pig)
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=3μg/Test ,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 34kDa
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse Nanog: 71-170/305 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human.
NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).

Function:
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation.

Subunit:
Interacts with SMAD1 and SALL4.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

Similarity:
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.

SWISS:
Q80Z64

Gene ID:
71950

Database links:

Entrez Gene: 79923 Human

Entrez Gene: 71950 Mouse

Omim: 607937 Human

SwissProt: Q9H9S0 Human

Unigene: 635882 Human



研究表明;早期胚胎在沒有Nanog存在的情況下是不能存活的,但是這一蛋白的表達水平至今還有待于進一步研究。學者認為:Nanog依然是一種對于生殖細胞(germ lls)形成的必要因子,Nanog也是調控著細胞融合后的多重功能的關鍵因子。
胚胎干細胞是當今生命科學和生物技術研究的熱點,這主要是由于其具有“發育全能性”的功能。ES能分化成人體200多種細胞類型,形成機體的任何細胞、組織和器官。通過掌握其分化發育的規律,在人工條件下定向分化為所需的細胞、組織乃至器官,科學家們希望可以用來治療目前還難以或無法治愈的帕金森氏病、早老性癡呆、白血病、糖尿病等頑癥,并且解決十分緊缺的組織和器官移植的來源問題,并且通過進一步與克隆技術相結合,運用體細胞核轉移技術來得到ES,還能解決細胞治療以及組織和器官移植的免疫排異難題。
產品圖片
Tissue/cell: Mouse tooth germ tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated(bs-0829R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti-Nanog antibody(bs-0829R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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