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Rabbit Anti-IRS3  antibody (bs-0186R)  
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產品編號 bs-0186R
英文名稱 Rabbit Anti-IRS3  antibody
中文名稱 胰島素受體底物-3抗體
別    名 IRS1--Insulin receptor substrate-3; IRS3; IRS-3; IRS3.  
Specific References  (1)     |     bs-0186R has been referenced in 1 publications.
[IF=3.996] Yanhan Ma. et al. Effect of Interleukin-1β on Gene Expression Signatures in Schwann Cells Associated with Neuropathic Pain. 2021 Jul 15  IHC ;  Rat.  
研究領域 細胞生物  神經生物學  信號轉導  激酶和磷酸酶  糖尿病  內分泌病  結合蛋白  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Mouse,Rat,Pig
產品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 53kDa
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse IRS-3: 261-360/494 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The family of insulin receptor substrates (IRSs) has been reported to play important roles for signal transduction of various hormones. Four members of the IRS family have been described. Each IRS is believed to have different functions; however, the distinct physiological roles of each IRS are unclear. IRS-1 may mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2.

SWISS:
O54718

Gene ID:
16369

Database links:


Entrez Gene: 16369 Mouse

SwissProt: O54718 Mouse





胰島素受體底物-3屬于IRS家族。IRS作為insulin/IGF 信號途徑的核心介子對細胞的生長、發育及新陳代謝的調節起關鍵作用。
產品圖片
Sample: Lane 1: Adipose (Rat) Lysate at 40 ug Primary: Anti-IRS3 (bs-0186R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRS3) Polyclonal Antibody, Unconjugated (bs-0186R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human pneumonia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IRS-3 Polyclonal Antibody, Unconjugated(bs-0186R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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