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Rabbit Anti-IFN gamma  antibody (bs-0480R)  
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產(chǎn)品編號(hào) bs-0480R
英文名稱(chēng) Rabbit Anti-IFN gamma  antibody
中文名稱(chēng) 干擾素-γ/IFN-γ抗體
別    名 IFNG; IFG; IFI; IFN Gamma; IFN Immune; IFN-gamma; IFNG; IFNG_MOUSE; Immune Interferon; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon.  
Specific References  (70)     |     bs-0480R has been referenced in 70 publications.
[IF=16.836] Leonard Siebert. et al. Light‐Controlled Growth Factors Release on Tetrapodal ZnO‐Incorporated 3D‐Printed Hydrogels for Developing Smart Wound Scaffold. 2021 Feb 19  IHC ;  Mouse.  
[IF=12.91] Kai Ye. et al. An armed oncolytic virus enhances the efficacy of tumor-infiltrating lymphocyte therapy by converting tumors to artificial antigen presenting cells in situ. MOL THER. 2022 Jun;:  IHC ;  Mouse.  
[IF=12.469] Weinan Guo. et al. Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis. J IMMUNOTHER CANCER. 2022 Jun;10(6):e004381  IF ;  Mouse.  
[IF=12.153] Hong Jihye. et al. Senescent cancer cell-derived nanovesicle as a personalized therapeutic cancer vaccine. EXP MOL MED. 2023 Mar;:1-14  WB ;  Mouse.  
[IF=11.467] Xudong Wen. et al. A macrophage membrane-coated mesoporous silica nanoplatform inhibiting adenosine A2AR via in situ oxygen supply for immunotherapy. J CONTROL RELEASE. 2023 Jan;353:535  IF ;  Mouse.  
[IF=8.786] Xiaoyu Guo. et al. Effects of Boric Acid Gel on Vaginal Candida albicans Infections and the Local Immune System in Mice. FRONT IMMUNOL. 2022; 13: 950215  IHC ;  Mouse.  
[IF=8.579] Tong?L et al. ACT001 reduces the expression of PD-L1 by inhibiting the phosphorylation of STAT3 in glioblastoma. Theranostics. 2020 May 1;10(13):5943-5956.  IHC ;  Mouse.  
[IF=8.136] Dupont L et al. Spontaneous atopic dermatitis due to immune dysregulation in mice lacking Adamts2 and 14.Matrix Biol. 2018 Sep;70:140-157.  IHC-P ;  Mouse.  
[IF=7.658] Yongbo Kang. et al. Lactobacillus acidophilus ameliorates obesity in mice through modulation of gut microbiota dysbiosis and intestinal permeability. Pharmacol Res. 2021 Dec;:106020  IHC ;  Mouse.  
[IF=7.324] Yongbo Kang. et al. A Novel Synbiotic Alleviates Autoimmune Hepatitis by Modulating the Gut Microbiota-Liver Axis and Inhibiting the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway. MSYSTEMS. 2023 Feb 16  IHC ;  Mouse.  
[IF=7.238] Chinthalapally V. Rao. et al. GSK3‐ARC/Arg3.1 and GSK3‐Wnt signaling axes trigger amyloid‐β accumulation and neuroinflammation in middle‐aged Shugoshin 1 mice. Aging Cell. 2020 Oct;19(10):e13221  IHC ;  Mouse.  
[IF=7.182] Tingting Guo. et al. Lepidium meyenii Walpers polysaccharide and its cationic derivative re-educate tumor-associated macrophages for synergistic tumor immunotherapy. Carbohyd Polym. 2020 Dec;250:116904  IF ;  Mouse.  
[IF=6.922] Maoyu Ye. et al. Long-Term Exposure to Sulfur Dioxide Before Sensitization Decreased the Production of Specific IgE in HDM-Sensitized Allergic Rhinitis Mice. J INFLAMM RES. 2022; 15: 2477–2490  IHC ;  Mouse.  
[IF=6.575] Qingqing Liu. et al. A Synbiotic Ameliorates Con A-Induced Autoimmune Hepatitis in Mice Through Modulation of Gut Microbiota And Immune Imbalance. MOL NUTR FOOD RES. 2023 Jan;:2200428  IF,IHC ;  Mouse.  
[IF=6.551] Gaolong Zhong. et al. Arsenic exposure induces intestinal barrier damage and consequent activation of gut-liver axis leading to inflammation and pyroptosis of liver in ducks. Sci Total Environ. 2021 Sep;788:147780  WB ;  Duck.  
[IF=6.304] Jun Tan. et al. Small molecules targeting RORγt inhibit autoimmune disease by suppressing Th17 cell differentiation. Cell Death Dis. 2020 Aug;11(8):1-14  WB ;  Mouse.  
[IF=6.048] Qingqing Liu. et al. Probiotics alleviate autoimmune hepatitis in mice through modulation of gut microbiota and intestinal permeability. J Nutr Biochem. 2021 Sep;:108863  IHC ;  mouse.  
[IF=5.988] Yang Liu. et al. Platycodon grandiflorus polysaccharides deeply participate in the anti-chronic bronchitis effects of platycodon grandiflorus decoction, a representative of "the lung and intestine are related". FRONT PHARMACOL. 2022 Sep 7;13:927384  ELISA ;  Rat.  
[IF=5.853] Soyeon Kim. et al. Defective N-glycosylation in tumor-infiltrating CD8+ T cells impairs IFN-γ-mediated effector function. IMMUNOL CELL BIOL. 2023 Apr;:  WB ;  Mouse.  
[IF=5.714] Mingwei Tong. et al. The Trichinella spiralis-derived antigens alleviate HFD-induced obesity and inflammation in mice. INT IMMUNOPHARMACOL. 2023 Apr;117:109924  IHC ;  Mouse.  
[IF=5.712] Li, Jing. et al. Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration. Cell Commun Signal. 2021 Dec;19(1):1-20  IF ;  Mouse.  
[IF=5.58] Zhang, Qian-Qian, et al. "CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment." Scientific reports 5 (2015).  WB ;  Mouse.  
[IF=5.41] Makar, Tapas K., et al. "Silencing of Abcc8 or inhibition of newly upregulated Sur1-Trpm4 reduce inflammation and disease progression in experimental autoimmune encephalomyelitis." Journal of Neuroinflammation 12.1 (2015): 1-13.  IHC-P ;  Mouse.  
[IF=5.34] Dian-Dong Hou. et al. Therapeutic effects of myricetin on atopic dermatitis in vivo and in vitro. PHYTOMEDICINE. 2022 Jul;102:154200  IHC ;  Mouse.  
[IF=5.19] Tang, Lixuan. et al. Curcumin antagonizes inflammation and autophagy induced by arsenic trioxide through immune protection in duck spleen. ENVIRON SCI POLLUT R. 2022 Jun;:1-12  WB ;  Duck.  
[IF=4.932] Shuling Zhang. et al. A novel mechanism of lung cancer inhibition by methionine enkephalin through remodeling the immune status of the tumor microenvironment. Int Immunopharmacol. 2021 Oct;99:107999  IHC ;  Mouse.  
[IF=4.925] Xizhan Xu. et al. Time-Course Transcriptomic Analysis Reveals the Crucial Roles of PANoptosis in Fungal Keratitis. INVEST OPHTH VIS SCI. 2023 Mar;64(3):6-6  IHC,IF ;  Mouse.  
[IF=4.36] An Wang. et al. Yiqi Jiedu herbal decoction attenuates the 2?Gy 60Co γ ray induced spleen injury by inhibiting apoptosis and modulating the immune balance. J Ethnopharmacol. 2022 Mar;286:114925  IHC ;  Mouse.  
[IF=4.178] Mariana Diupotex. et al. Alpha-galactosylceramide as adjuvant induces protective cell-mediated immunity against Leishmania mexicana infection in vaccinated BALB/c mice. CELL IMMUNOL. 2023 Feb;:104692  IHC ;  Mouse.  
[IF=4.12] Zhou, Xue, et al. "Sulindac has strong antifibrotic effects by suppressing STAT3‐related miR‐21." Journal of Cellular and Molecular Medicine (2015).  WB ;  Human.  
研究領(lǐng)域 腫瘤  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  干擾素  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=0.5ug/Test,ICC/IF=1:50,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 15kDa
細(xì)胞定位 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human IFN gamma: 1-100/166 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.

Function:
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Subunit:
Homodimer.

Subcellular Location:
Secreted.

Tissue Specificity:
Released primarily from activated T lymphocytes.

Post-translational modifications:
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

DISEASE:
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

Similarity:
Belongs to the type II (or gamma) interferon family.

SWISS:
P01580

Gene ID:
15978

Database links:

Entrez Gene: 3458 Human

Entrez Gene: 15978 Mouse

Entrez Gene: 360697 Rat

Omim: 147570 Human

SwissProt: P01579 Human

SwissProt: P01580 Mouse

Unigene: 856 Human

Unigene: 240327 Mouse



產(chǎn)品圖片
Sample: Disease Lung (Mouse) Lysate at 30 ug Disease Spleen (Mouse) lysate at 30 ug Primary: Anti- IFN gamma (bs-0480R) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution Predicted band size: 15 kD Observed band size: 18 kD
Sample: Lane 1: Recombinant human IFN Gamma protein, N-His Tag Primary: Anti-IFN gamma (bs-0480R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 16 kDa
Sample: Lane 1: Mouse Raw264.7-PMA Lysates Lane 2: Mouse Lymph Lysates Lane 3: Mouse Spleen Lysates Lane 4: Mouse Thymus Lysates Lane 5: Rat Lymph Lysates Lane 6: Rat Thymus Lysates Lane 7: Human Jurkat-TPA cell Lysates Lane 8: Human Jurkat cell Lysates Lane 9: Human HepG2 cell Lysates Lane 10: Recombinant human IFN gamma Protein (bs-0388P) Primary: Anti-IFN gamma (bs-0480R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15kDa Observed band size: 25kDa
Tissue/cell: Mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IFN gamma Polyclonal Antibody, Unconjugated(bs-0480R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IFN-gamma Polyclonal Antibody, Unconjugated(bs-0480R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
ctll-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (IFN gamma) polyclonal Antibody, Unconjugated (bs-0480R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (mouse lymph); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat lymph); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IFN gamma) Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Blank control(black line):ctll-2. Primary Antibody (green line): Rabbit Anti-IFN gamma antibody (bs-0480R) Dilution:0.5ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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