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Rabbit Anti-CD34  antibody (bs-0646R)  
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產品編號 bs-0646R
英文名稱 Rabbit Anti-CD34  antibody
中文名稱 CD34抗體
別    名 CD34 antigen; CD34 molecule; Cluster designation 34; Hematopoietic progenitor cell antigen CD34; HPCA1; CD34_HUMAN.  
Specific References  (51)     |     bs-0646R has been referenced in 51 publications.
[IF=11.322] Zhenyin Chen. et al. Gelatin/sodium alginate composite hydrogel with dynamic matrix stiffening ability for bone regeneration. COMPOS PART B-ENG. 2022 Aug;243:110162  IF ;  Rat.  
[IF=10.652] Pei Liu. et al. Biphasic CK2.1-coated β-glycerophosphate chitosan/LL37-modified layered double hydroxide chitosan composite scaffolds enhance coordinated hyaline cartilage and subchondral bone regeneration. Chem Eng J. 2021 Aug;418:129531  IHC ;  Rabbit.  
[IF=10.652] Zhuo Liang. et al. Exosome derived from mesenchymal stem cells mediates hypoxia-specific BMP2 gene delivery and enhances bone regeneration. Chem Eng J. 2021 Oct;422:130084  IF ;  Rabbit.  
[IF=9.776] Ruijing Zhao. et al. Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury. J Control Release. 2022 May;345:214  FC ;  Human.  
[IF=8.786] Zhaoqi Zhang. et al. Central gene transcriptional regulatory networks shaping monocyte development in bone marrow.. FRONT IMMUNOL. 2022 Oct;13:1011279-1011279  FC ;  Mouse.  
[IF=7.328] Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359  IF ;  Mouse.  
[IF=7.129] Huifeng Yue. et al. Maternal NO2 exposure disturbs the long noncoding RNA expression profile in the lungs of offspring in time-series patterns. ECOTOX ENVIRON SAFE. 2022 Nov;246:114140  IHC ;  Mouse.  
[IF=6.6] Hsueh-Chun Wang. et al. Restoring Osteochondral Defects through the Differentiation Potential of Cartilage Stem/Progenitor Cells Cultivated on Porous Scaffolds. Cells-Basel. 2021 Dec;10(12):3536  FC ;  Human.  
[IF=5.579] Chen et al. Lipoxin A4 and its analogue suppress the tumor growth of transplanted H22 in mice: the role of antiangiogenesis. (2010) Mol.Cancer.The. 9:2164-74  IHC-F ;  Mouse.  
[IF=5.546] Huang Z et al. The impact of acute stress disorder on gallbladder interstitial cells of Cajal. J Cell Physiol . 2020 Apr 24.  WB,IF ;  rabbit.  
[IF=5.395] Zhenyin Chen. et al. Zonally Stratified Decalcified Bone Scaffold with Different Stiffness Modified by Fibrinogen for Osteochondral Regeneration of Knee Joint Defect. ACS BIOMATER-SCI ENG. 2022;XXXX(XXX):XXX-XXX  IF ;  Rabbit.  
[IF=5.135] Ci Li. et al. Sustained release of exosomes loaded into polydopamine-modified chitin conduits promotes peripheral nerve regeneration in rats. Neural Regen Res. 2022 Feb;17(9):2050  FC ;  Rat.  
[IF=4.982] Yuan, Liwei. et al. Bone marrow mesenchymal stem cells combined with estrogen synergistically promote endometrial regeneration and reverse EMT via Wnt/β-catenin signaling pathway. REPROD BIOL ENDOCRIN. 2022 Dec;20(1):1-13  FC ;  Rabbit.  
[IF=4.963] Jin J et al. Exosome?secreted?from?adipose-derived?stem?cells?attenuates?diabetic?nephropathy?by?promoting?autophagy?flux?and?inhibiting?apoptosis?in?podocyte.Stem?Cell Res Ther.?2019 Mar 15;10(1):95.  ICF ;  Human.  
[IF=4.963] Chen X et al. Transplantation of oral mucosal epithelial cells seeded on decellularized and lyophilized amniotic membrane for the regeneration of injured endometrium.Stem Cell Res Ther. 2019 Mar 21;10(1):107.  IHC-P ;  Rat.  
[IF=4.648] Long et al. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. (2017) Aging.Di. 8:301-313  FCM ;  Rat.  
[IF=4.627] Zhai L et al. Aerobic and resistance training enhances endothelial progenitor cell function via upregulation of caveolin-1 in mice with type 2 diabetes. Stem Cell Res Ther. 2020 Jan 3;11(1):10.  ICF ;  Mouse.  
[IF=4.61] Yue H et al. Gestational exposure to PM2.5 impairs vascularization of the placenta. Sci Total Environ. 2019 May 15;665:153-161.  IHC-P&WB ;  Mouse.  
[IF=4.47] Erin, Nuray, et al. "Differential characteristics of heart, liver, and brain metastatic subsets of murine breast carcinoma." Breast Cancer Research and Treatment (2013): 1-13.  Mouse.  
[IF=4.197] Xuewen Qian. et al. Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a. Lab Invest. 2020 Aug;101(3):280-291  IHC ;  Human.  
[IF=3.998] Gae Won Parket al. Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns. Sci Rep . 2020 Jul 24;10(1):12448.  FCM ;  rat.  
[IF=3.923] Sha Y et al. MGF E peptide improves anterior cruciate ligament repair by inhibiting hypoxia‐induced cell apoptosis and accelerating angiogenesis.(2018) J Cell Physiol. 2018 Oct 14.  IF ;  Rabbit.  
[IF=3.887] Yan LI. et al. The combination of EGCG with warfarin reduces deep vein thrombosis in rabbits through modulating HIF-1α and VEGF via the PI3K/AKT and ERK1/2 signaling pathways. CHIN J NAT MEDICINES. 2022 Sep;20:679  IF ;  Rabbit.  
[IF=3.752] Lifen Wang. et al. Electroacupuncture attenuates ischemic injury after stroke and promotes angiogenesis via activation of EPO mediated Src and VEGF signaling pathways. PLOS ONE. 2022 Sep;17(9):e0274620  WB ;  Rat.  
[IF=3.715] Nan Hou. et al. Tissue-engineered esophagus: recellular esophageal extracellular matrix based on perfusion-decellularized technique and mesenchymal stem cells. Biomed Mater. 2021 Aug;:  IHC ;  Rabbit.  
[IF=3.485] Bai J et al. Irradiation-induced senescence of bone marrow mesenchymal stem cells aggravates osteogenic differentiation dysfunction via paracrine signaling. Am J Physiol Cell Physiol . 2020 May 1;318(5):C1005-C1017.  FCM ;  rat.  
[IF=3.427] Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900  FCM ;  Human.  
[IF=3.352] Qi Wang. et al. Telocytes in The Esophageal Wall of Chickens: A Tale of Subepithelial Telocytes. Poultry Sci. 2022 Mar;:101859  IHC ;  Chicken.  
[IF=3.263] Mahmoud E. Youssef. et al. α7-nAChRs-mediated therapeutic angiogenesis accounts for the advantageous effect of low nicotine doses against myocardial infarction in rats. Eur J Pharmacol. 2021 Mar;:173996  IHC ;  Rat.  
[IF=2.976] Guo H et al. Clinical associations between ASCT2 and p-mTOR in the pathogenesis and prognosis of epithelial ovarian cancer. Oncol Rep. 2018 Dec;40(6):3725-3733.  IHC-P ;  Human.  
研究領域 腫瘤  心血管  免疫學  發育生物學  神經生物學  干細胞  細胞表面分子  糖蛋白  細胞類型標志物  血管內皮細胞  內皮細胞  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Mouse,Rat
產品應用 WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,IP=1:20-50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 39kDa
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD34: 301-385/385 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.

Function:
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Post-translational modifications:
Highly glycosylated.
Phosphorylated on serine residues by PKC.

Similarity:
Belongs to the CD34 family.

SWISS:
P28906

Gene ID:
947

Database links:

Entrez Gene: 947 Human

Omim: 142230 Human

SwissProt: P28906 Human

Unigene: 374990 Human



造血干細胞標志物
內皮標志物
腫瘤生物標志物
細胞表面的唾液粘蛋白。
間充質干細胞(mesenchymal stem cells,MSC)也是干細胞家族的重要成員,來源于發育早期的中胚層和外胚層。MSC最初在骨髓中發現,因其具有多向分化潛能、造血支持和促進干細胞植入、免疫調控和自我復制等特點。如間充質干細胞在體內或體外特定的誘導條件下,可分化為血管內皮、脂肪、骨、軟骨、肌肉、肌腱、韌帶、神經、肝、心肌、等多種組織細胞。
產品圖片
25 ug total protein per lane of various lysates (see on figure) probed with CD34 polyclonal antibody, unconjugated (bs-0646R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (bs-0646R) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.
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